Reverse-Phase Protein Microarrays

The Reverse Phase Protein Array (RPPA) is a flexible, high-density protein lysate microarray that enables sensitive, non-subjective, quantitative, calibrated, multiplexed analysis of cellular proteins from a limited amount of starting sample, such as small cell culture samples or clinical specimens.

Key Advantages

When compared to other commonly used proteomic analysis platforms (e.g. Western blot, ELISA or Mass Spec), the RPPA platform offers significant advantages in terms of its sensitivity, sample throughput, data consistency and speed.

For example, the RPPA platform can be several orders of magnitude more sensitive than technologies such as an ELISA or Western blot; the amount of protein that is typically loaded into one lane for Western blot analysis is enough to perform hundreds to thousands of RPPA assays whose protein lysate requirements are generally in the picogram to nanogram range. Moreover, hundreds of different protein lysate samples can be analyzed simultaneously under identical assay conditions in the presence of analyte controls and calibration standards.

Further Information on RPPA Assays

For more details on how the RPPA process works, please link to Proteomic Process.

For a listing of currently available RPPA assays, please select Available RPMA Assays (October 2014)

For further information, please contact Theranostics Health directly with your inquiries.

Technology

Proteomic Process Overview

Laser Capture Microdissection

Pathways and Disease

Publication Index

Reverse-Phase Protein Microarrays

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