How It Works
Nanoliter amounts of cell lysates (e.g. cell culture, animal model tissues, clinical biopsies, immune cells isolated from whole blood, Laser Capture Microdissection samples, etc.) are precision-spotted, or printed, on a nitrocellulose-coated microarray slide. Due to the small diameter of each spotted sample, Theranostics Health is able to print hundreds of different experimental or clinical samples in technical replicates on each slide in the presences of positive controls and calibration standards.
To detect a protein of interest, an individual microarray slide is first stained with a primary antibody that is specific to the analyte of interest, and then is subsequently stained with a fluorescently-labeled secondary antibody. To perform a signal transduction pathway analysis experiment, multiple microarray slides are prepared and a separately stained with antibodies that are specific to the proteins and/or phosphorylated proteins that belong to the signaling pathway(s) of interest.
Fluorescently-stained microarray slides are analyzed using an array laser scanning apparatus and the resulting image is converted into a numerical data format that can be analyzed using commonly accepted bioinformatic methods.