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Nature Technology Feature: Protein Arrays
The reverse phase microarray is…an alternative to conventional 'capture arrays' where antibodies are spotted onto a substrate and exposed to sample – and where problems such as high signal-to-noise ratio arise… "After encountering limited success with capture arrays in their clinical research, Liotta and Petricoin developed the reverse-phase protein array, in which samples are spotted on the array and then probed with detection antibodies, requiring only one antibody per analyte. Much of their work has centered on signaling pathways in human disease, and this technique has worked well with their research, they say. "On the clinical side, Liotta and Petricoin have found success using phosphospecific antibodies with reverse-phase arrays to characterize differential phosphorylation in biopsy specimens, and their arrays are now undergoing clinical trials as a diagnostic or prognostic tool for cancer." "From a few thousand cells obtained by laser microdissection, we can look at hundreds of phosphorylation endpoints quantitatively, and look at a target and all the downstream signal-ling around it," says Liotta.
Article: Protein arrays: Growing pains |
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